aliquoted

Genital fluids from 109 subjects, including male and female cohorts from Italy and Cambodia, were collected, aliquoted and processed with different techniques, to assess optimal conditions maximizing mucosal antibody recovery.

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The samples were immediately aliquoted and frozen at − 80°C.

PLoS ONE Alerts: New Articles Pramod K. Dash et al. 2010

The samples were centrifuged for 10 min at 4C and the resulting plasma aliquoted into Eppendorf tubes, which were frozen immediately at − 80°C.

PLoS ONE Alerts: New Articles Enrico Domenici et al. 2010

Each reagent kit includes pre-aliquoted reagents and primers needed for synthesizing first-strand cDNA ready for preamplification and pathway-focused analysis.

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The samples were centrifuged for 10 min at 4C and the resulting plasma aliquoted into Eppendorf tubes, which were frozen immediately at − 80°C.

PLoS ONE Alerts: New Articles Enrico Domenici et al. 2010

Primed and control cells were then aliquoted into designated small tissue culture flasks (25 ml volume) and treated with 50 µM of noscapine or its analogs or DMSO for 2 hr or instantly prior to triggering oxidative burst.

PLoS ONE Alerts: New Articles Susu Zughaier et al. 2010

This final mixture was aliquoted into four 2 mL cryotubes which were flash-frozen and returned to − 80°C.

PLoS ONE Alerts: New Articles Jenna L. Morgan et al. 2010

Pure fractions were dialyzed (20 mM Tris-Cl [pH 8.0], 10% glycerol and 150 mM NaCl), aliquoted and stored at − 80°C.

PLoS ONE Alerts: New Articles Gunjan Arora et al. 2010

Immediately after counting, the cultures were aliquoted into ten 2 mL cryotubes, flash-frozen in liquid nitrogen and stored at − 80°C.

PLoS ONE Alerts: New Articles Jenna L. Morgan et al. 2010

For each peptide or protein, an amount sufficient for inseminating 32 animals (two times 16) with 0.33 prostate gland equivalents each was aliquoted and put on ice.

PLoS ONE Alerts: New Articles Joris M. Koene et al. 2010