hMSC

In Quantitative monitoring of hMSC intrinsic fluorescence.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

To our knowledge, this work represents the first application of MPM in which long term monitoring of hMSC differentiation reveals quantitative changes in cellular metabolic activity (via computed redox ratio) and oxidative stress (via lipofuscin) using entirely endogenous sources of contrast.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

As described in the materials and methods, TPEF images excited at 755 nm and 860 nm are acquired from the same hMSC cultures over 16-21 days and analyzed for changes in NAD (P) H, lipofuscin and FP levels.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

To identify the components of the hMSC intrinsic cellular fluorescence, we collected and analyzed TPEF emission spectra, establishing the spectral shapes of the contributing fluorophores.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

(A) hMSC TPEF emission spectra collected at either 755 nm or 860 nm excitation with corresponding fits by the ALS script.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

In addition, one can conclude that maintaining hMSC cultures at 5% oxygen leads to a reduction in the rate of lipofuscin accumulation as we do not observe continued proliferation from hMSCs under hypoxic conditions.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

Many factors influence hMSC proliferation and differentiation, including soluble factors added to the culture medium

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

If lipofuscin is indeed formed in response to cellular ROS, it is possible that isolation and maintenance of hMSC at 20% oxygen leads to premature aging, accumulation of defective mitochondria and subsequent lipofuscin formation, while the cells themselves are producing ATP via glycolysis.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

In this study, we demonstrate the utility of MPM imaging and spectroscopy as non invasive tools for monitoring hMSC cultures.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

Volkmer E, Kallukalam BC, Maertz J, Otto S, Drosse I, et al. (2009) Hypoxic preconditioning of hMSC overcomes hypoxia-induced inhibition of osteogenic differentiation.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010