immunoprecipitate

These experiments revealed that dMfn co-immunoprecipated with Parkin in wt flies, but failed to detect a dMfn band in an immunoprecipitate from Affinity purified anti-Parkin antiserum was used to immunoprecipitate Parkin from wt flies and park25 null mutants.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

We observed that in both human and mouse cardiomyocytes, BIN1 and Cav1. 2 colocalize at cardiac T-tubules (by fluorescence and electron microscopy immunogold labeling) and co-immunoprecipitate.

PLoS Biology: New Articles Ting-Ting Hong et al. 2010

The anti-ubiquitin antiserum detected a band of approximately 120 kDa in the immunoprecipitate from wt flies but detected only a very faint

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

Affinity purified anti-dMfn antiserum was used to immunoprecipitate dMfn from wild-type flies,

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

To control for the specificity of our anti-dMfn antiserum, we also immunoprecipated dMfn from flies expressing an RNAi construct targeting the dMfn transcript and subjected the immunoprecipitate to western blot analysis with an anti-dMfn and an anti-ubiquitin antiserum.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

In the course of testing whether a commercial rabbit antiserum to human Mitofusin 2 (Sigma (St. Louis, MO) #M6319) would cross-react with the corresponding fly protein, this antiserum was used to immunoprecipitate proteins from an adult fly protein extract and the immunoprecipitate was subjected to tandem mass spectrometry analysis.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

To help distinguish between these models we immunoprecipitated Parkin from wt flies and subjected the immunoprecipitate to western blot analysis with an anti-dMfn antiserum.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

As a control for specificity, anti-dMfn antiserum was also used to immunoprecipitate dMfn from flies with hsp70-GAL4 driven expression of UAS-dmfn-RNAiVienna.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

An anti-dMfn antiserum generated against the peptide DTVDKSGPGSPLSRF was provided by Dr. Alex Whitworth and used to immunoprecipitate dMfn from wt flies, PINK1 [B9] mutants, and park

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

To control for the possible nonspecific interaction of dMfn with our anti-Parkin antiserum, we also subjected a protein extract from park25 null mutants to immunoprecipitation with an anti-Parkin antiserum and then used this immunoprecipitate in a western blot analysis with our anti-dMfn antiserum.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010