normoxia

(A) Western blot analysis of HIF-1α and bcl-2 protein expression in total extracts of M14 cells transfected with siRNA targeting bcl-2 mRNA (si-bcl-2) or with a control scrambled si-RNA (si-contr) and then exposed to normoxia or hypoxia for 24 h.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

(A) Western blot analysis of HSP90α and HSP90β protein expression in M14 control (puro) and bcl-2 stably overexpressing (Bcl2/5, Bcl2/37) clones exposed to hypoxia or to normoxia for 24

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Benizri E, Ginouves A, Volmat V, Roux D, et al. (2003) HIF prolyl-hydroxylase 2 is the key oxygen sensor setting low steady-state levels of HIF-1alpha in normoxia.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

(B) Confocal laser scanning microscopy of immunofluorescence staining performed on Bcl2/5 stably overexpressing clone exposed to hypoxia or to normoxia for 24 h.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

(Bcl2/5) clones exposed to hypoxia or to normoxia for 24 h.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

To evaluate whether down-regulation of bcl-2 shows opposite effect of bcl-2 overexpression in terms of HIF-1α protein expression, we silenced the endogenous expression of bcl-2 gene transfecting M14 cells with siRNA-targeting bcl-2 mRNA (si-bcl-2) and then exposing them to normoxia or hypoxia for 24 h.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

COL 1a1 expression on day 8 was significantly higher under hypoxic conditions than it was in normoxia while on day 16 expression in the normoxic group was significantly higher than that at 5% oxygen.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

Cells in osteogenic differentiation medium at 5% and 20% oxygen exhibit an initial decrease in the redox ratio on day 4, followed by a consistent increase, with the mean values achieving statistically similar values to those of day 0, by day 8 (in the case of normoxia) or day 12 (in the case of hypoxia).

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

Day 4 represents the only time point at which hMSCs from the hypoxia propagation group (HP) have greater lipofuscin levels than those maintained in normoxia.

PLoS ONE Alerts: New Articles William L. Rice et al. 2010

Although under normoxia this HIF-1α stabilization is not sufficient to affect the steady state levels of the protein, it becomes rate limiting during hypoxia or, in general, in conditions strictly dependent on oxygen level.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010