Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun a
polyribosome
Etymologies
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Examples
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ZNF9 and GAPDH western blotting of supernatant and polysome fractions of HeLa cell lysates isolated in the absence or presence of EDTA.
PLoS ONE Alerts: New Articles Morgan A. Sammons et al. 2010
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Indeed, the vast majority of gemin5, whether as free protein or bound to the SMN complex, is present in translationally inactive fractions of polysome gradients
PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009
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Indeed, the miRNA-associated protein Ago2 was reported to repress mRNA translation through direct interaction with the 5′ cap HeLa and 293T cell lysates were prepared using polysome lysis buffer (PLB) as described
PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009
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In a similar fashion, polysome profiles were determined for T. brucei
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Similar to what we describe here, there is a comparable reduction in polysome abundance after the induction of a heat-shock response in trypanosomes, yet no evidence for changes in the phosphorylation state of eIF2α
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Cells were resuspended in an equal volume of polysome lysis buffer
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Cells were resuspended in polysome buffer (120 mM KCl, 2 mM MgCl
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We are very grateful to Elisabetta Ullu (Yale University) for discussions and advice concerning the polysome analysis, Beatriz Castilho and Osvaldo Neto (University of São Paulo,
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