sonicate

M. tuberculosis-sonicate antigens increased specific luciferase activity in a dose-dependent manner and to a level greater than that induced by

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

M. tuberculosis-sonicate was used to stimulate the cells for the experiments shown in B, C, and D.

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

M. tuberculosis-sonicate antigen stimulated (+) THP-1 cells that were cultured for 48 hours in serum-free media with or without increasing amounts of human rMCP-1.

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

M. tuberculosis-sonicate antigens plus rMCP-1 was always significantly greater than in that culture media from cells stimulated with antigen alone.

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

Cells were stimulated overnight with or without M. tuberculosis-sonicate antigens and with the indicated amounts of human recombinant MCP-1.

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

M. tuberculosis-sonicate plus the various concentrations of rMCP-1 than in cells stimulated with

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010

The amount of sonicate added per flask of stimulated cells originated from one flask of RTHDF fibroblasts.

PLoS ONE Alerts: New Articles Hans-Christian Ingerslev et al. 2010

The sonicate was then centrifuged for 2 min at 14. 000× g and the clear supernatant was transferred to a new tube.

PLoS ONE Alerts: New Articles Hans-Christian Ingerslev et al. 2010

The sonicate was prepared by an initial trypsination of confluent cell culture flasks followed by sonication for 30 s (amplitude 10 microns) using a MSE Soniprep 150 sonicator (Sanyo).

PLoS ONE Alerts: New Articles Hans-Christian Ingerslev et al. 2010

M. tuberculosis-sonicate antigens alone or in the presence of human rMCP-1.

PLoS ONE Alerts: New Articles Malathesha Ganachari et al. 2010